我的研究方向是溶酶体相关细胞器(LROs)的生物发生。. LROs存在于人体多种细胞类型中, 包括黑色素细胞(头发中产生色素的细胞), skin and eyes), lung epithelial cells, platelets and immune cells. In each of these cell types, LROs coexist with classical lysosomes, indicating that cells producing LROs have additional trafficking machinery to route cargoes towards LROs and away from classical lysosomes; however, 人们对这种贩运机制还不完全了解. Additionally, LRO生物发生缺陷导致许多疾病, including Hermansky-Pusdlak, Griscelli and Chediak-Higashi syndromes. In 2019, a R15基金被授予推进本科研究 at Marist.

在实验室中，我们使用永生化小鼠黑素细胞作为研究LRO生物发生的模型系统. 这些细胞适用于一系列细胞生物学技术, ranging from microscopy to biochemistry, 我们有Hermansky-Pudlak综合征小鼠模型的细胞系来研究LRO生物发生中单个蛋白质的作用. Additionally, 当LRO生物发生受损时，细胞会发生显著的表型变化——从色素沉着转变为低色素沉着——这为LRO生物发生的新型介质的表型筛选提供了一种快速机制. 

Recently, 我的研究已经扩展到包括鉴定和研究新的噬菌体通过 SEA-PHAGES program, in collaboration with the University of Pittsburgh & the Howard Hughes Medical Institute. 噬菌体是感染不同类型细菌宿主的微小病毒，已在医疗和农业环境中用于控制细菌感染，包括已产生抗生素耐药性的细菌感染. 因为大多数类型的噬菌体只感染一小部分细菌宿主, 这种类型的噬菌体疗法/治疗可以消除有害细菌，同时保持有益微生物群落的完整. Working with first-year students at Marist, 我们从校园周围的地点分离出新型噬菌体，并对其形态和遗传特征进行了表征. In the future, students will continue to characterize their phage, 包括研究了解每种噬菌体可以感染和杀死的细菌宿主类型. 

Bowman SL, Le L, Zhu Y, Sitaram A, Tenza D, Sviderskaya EV, Bennett DC, Bonifacino JS, Raposo G, Owen DJ, Dennis MK, and Marks MS. (2021) block -1: AP-3超复合物将cis-SNARE复合物分类为可循环的内体管状运输载体. Journal of Cell Biology, 220(7): e202005173. doi: 10.1083/jcb.202005173. Epub 2021 April 22.

Le L, Escobar IE, Ho T, Lefkovith AJ, Latteri E, Dennis MK, Haltaufderhyde KD, Sviderskaya EV, Bennett DC, Oancea E, and Marks MS. (2020) SLC45A2蛋白稳定性及调节黑素小体pH决定黑素细胞色素沉着. Mol Biol Cell, 32(24): 2687-2702; doi: 10.1091/mbc.E20-03-0200

Ripoll L, Heiligenstein X, Hurbain I, Domingues L, Figon F, Petersen KJ, Dennis MK, Houdusse A, Marks MS, Raposo G, and Delevoye C. (2018) Myosin VI和分支肌动蛋白丝介导黑素体小管载体的膜收缩和裂变. Journal of Cell Biology, 217(8): 2709-2726. doi: 10.1083/jcb.201709055. Epub 2018 Jun 6.

Dennis MK, Delevoye C, Acosta-Ruiz A, Hurbain I, Romao M, Hesketh GG, Goff PS, Sviderskaya EV, Bennett DC, Luzio JP, Galli T, Owen DJ, Raposo G, and Marks MS. (2016) block -1和block -3通过不同的管状运输载体调节VAMP7往返于黑素体的循环. Journal of Cell Biology, 214(3): 293-308. doi:  10.1083/jcb.201605090.

Delevoye C, Heiligenstein X, Ripoll L, Gilles-Marsens F, Dennis MK, Linares RA, Derman L, Gokhale A, Morel E, Faundez V, Marks MS, and Raposo G. (2016) block -1将肌动蛋白和微管细胞骨架结合在一起，产生可循环的内体. Current Biology. 26(1):1-13.  doi: 10.1016/j.cub.2015.11.020. Epub 2015 Dec 24.

Dennis MK*, Mantegazza AR*, Snir OL, Tenza D, Acosta A, Delevoye C, Zorger R, Sitaram A, de Jesus-Rojas W, Ravichandran K, Rux J, Sviderskaya EV, Bennett DC, Raposo G, Marks MS, and Setty SRG. (2015) block -2的目标是将内体小管循环到黑素体以进行货物输送. Journal of Cell Biology, 209(4): 563-577. doi: 10.1083/jcb.201410026. *Contributed equally

*Petrie WK， *Dennis MK, Hu C, Dai D, Arterburn JB, Smith HO, Hathaway HJ, Prossnitz ER. (2013) G蛋白偶联雌激素受体(GPER)选择性配体调节子宫内膜肿瘤生长. Obstetrics and Gynecology International, vol. 2013, Article ID 472720. doi:10.1155/2013/472720. *Contributed equally.

Burai R, Ramesh C, Nayak TK, Dennis MK, Bryant BK, Prossnitz ER, Arterburn JB. (2012)靶向G蛋白偶联雌激素受体GPER/GPR30的三羰基- re /Tc(I)螯合探针的合成与表征. PLoS One, 7(10):e46861. doi: 10.1371/journal.pone.0046861. Epub 2012 Oct 15.

Sitaram A, Dennis MK, Chaudhuri R, De Jesus-Rojas W, Tenza D, Setty SR, Wood CS, Sviderskaya EV, Bennett DC, Raposo G, Bonifacino JS, Marks MS. (2012) AP-1和AP-3对基于二苯甲酸的分选信号的差异识别揭示了OCA2向黑素体传递时block -1和AP-3都需要. Mol. Biol. Cell, 23(16): 3178-92. Epub 2012 June 20.

Nayak TK, Dennis MK, Ramesh C, Burai R, Atcher RW, Sklar LA, Norenberg JP, Hathaway HJ, Arterburn JB and Prossnitz ER. (2010)电荷对gpr30靶向(111)in标记非甾体显像剂细胞通透性和肿瘤成像的影响. ACS Chem Biol., 16;5(7): 681-90. Epub 3 June 2010. 

Ramesh C, Nayak TN, Burai R, Dennis MK, Hathaway HJ, Sklar LA, Prossnitz ER, Arterburn JB. (2010)靶向G蛋白偶联雌激素受体GPR30的碘化四氢喹啉类药物的合成与表征. J Med Chem. 53(3): 1004-1014. Epub 30 December 2009.

Dennis MK, Burai R, Ramesh C, Petrie WK, Alcon SN, Nayak TK, Bologa CG, Leitao A, Brailoiu E, Deliu E, Dun NJ, Sklar LA, Hathaway HJ, Arterburn JB, Oprea TI, Prossnitz ER. (2009) In vivo effects of a GPR30 antagonist. Nature Chemical Biology. 5(6): 421-427.

Blasko E, Haskell CA, Leung S, Gualtieri G, Halks-Miller M, Mahmoudi M, Dennis MK, Prossnitz ER, Karpus WJ, Horuk R. (2009) GPR30激动剂G-1在多发性硬化症动物模型中的有益作用. J. Neuroimmunology. 214(1-2): 67-77. Epub 5 August 2009.

Dennis MK, Bowles HJ, MacKenzie DA, Burchiel SW, Edwards BS, Sklar LA, Prossnitz ER, Thompson TA. (2008)使用高通量Hyper-Cyt®流式细胞术系统进行多功能雄激素受体筛选(MARS)试验. Cytometry A. 73(5): 390-399.

2021-2024年美国国立卫生研究院学术研究增强奖(R15):染色质介导的生殖细胞基因组完整性维持. NICHD 1R15HD103115; $346,418.

2019-2023美国国立卫生研究院学术研究增强奖(R15): AP-3在黑色素小体生物发生中蛋白质分选和自噬中的作用. NIGMS 1R15GM132810; $379,914. 

2012-2015 Ruth L. Kirschstein国家研究服务奖(NRSA)博士后奖学金. NIAMS 1F32AR062476.

2010-2012宾夕法尼亚大学博士后研究机会 & Teaching (Penn-PORT) Postdoctoral Fellowship. 美国国立卫生研究院机构研究和学术职业发展奖(IRACDA). NIGMS K12GM081259.